图片展示

HuiBaiYi  New Materias

客服电话:

13319518603

English
  • English
  • 中文
图片展示
图片展示

News

Contact Us

General manager:
+86-731-85525705
+86-13319528603
Fax:+86-731-85525705
E-mail:ivy@hnhbsj.com
Factory Address: Huaihua City, Hunan Province Hongjiang District Industrial Park on the 8th

News Category

Details


Hunan yunbang: how to detect and extract curcumin

Author:Hunan Hui Bai Yi new materias Co; Ltd Click: Time:2019-12-05 00:00:00

Curcumin is a chemical component extracted from the rhizome of some plants in the family coronaceae and araceae. Curcumin contains about 3% ~ 6%.


What are the detection methods for curcumin:

Conventional methods:

The ethanol solution of sample A should show pure yellow and light green fluorescence.If the ethanol extract is added with concentrated sulfuric acid, it will produce a deep purple color.Chromogenic reaction B an aqueous solution or dilute ethanol solution of the sample treated with hydrochloric acid until slightly orange at the beginning.The mixture is divided into two parts, one with a small amount of boric acid powder or crystal, and the part without boric acid turns significantly redder than the part without boric acid.Several pieces of filter paper can also be impregnated in the ethanol solution of pigment, dried at 100℃, and then moistened with a dilute solution of boric acid (with a few drops of hydrochloric acid).It should be cherry red when dry.


Chromatographic detection method:

5 ml of the test solution (0.01g sample dissolved in 1ml95% ethanol) was dripped on the TLC (microcrystalline cellulose, 0.1mm) and placed in the expansion tank containing 3-methyl-1-butanol/ethanol/water/ammonia (4:4:2:1) mixture, so that the solvent front increased by 10 ~ 15 cm.After a day and night observation under ultraviolet light: there are three yellow spots, Rf between 0.2 ~ 0.4;Rf of other spots is 0.6 ~ 0.8.All spots fluoresced yellow under ultraviolet light.6.95% ethanol has the maximum absorption peak at 425 nm.


Extraction process of curcumin:

The extraction methods of curcumin are various and have their own characteristics in the technological process, but generally speaking, curcumin can be extracted and refined.

The yield of curcumin from turmeric was increased by 8.1% compared with the traditional extraction method.The extraction process conditions were as follows: enzymatic hydrolysis temperature: 50℃; pH4.5; time: 120 min; enzyme concentration: 0.35 mg/ml.

HPLC determination method, curcumin extraction quantity as the index, orthogonal test method was used to optimize the extraction process, and it was concluded that the optimal extraction technology of curcumin by ultrasonic method was to add 8 times of alkaline water with pH value of 12, and extract for 4 times, each time 40 min.


The material came from the Internet.

Want to learn more knowledge, biochemical reagent attention please "hunan rhyme state biological pharmaceutical co., LTD." the official website: http://www.hunanyunbang.com/

(all contents of this website, where indicated source is "hunan yunbang", the copyright belongs to hunan yunbang, without authorization, any media, websites or individuals shall not be reproduced, otherwise will be prosecuted for legal responsibility, authorized reproduction must indicate the source: "hunan yunbang".This net indicates the content that the source is other media is to reprint, reprint is for the viewpoint share only, copyright returns original author all, if have infringe copyright, please contact us promptly.


Contact No.:+0731-85525705   +86-13319518603

E-mail: ivy@hnhbsj.com     

Factory Address:  No.8, Hongjiang Industrial Park, Huaihua, Hunan, China

 

Sweep away us

Copyright(C)Hunan Huibaiyi New Materials Co., Ltd. All rights reserved 湘ICP备2022015278号

Service Center
热线电话
400-12346578
上班时间
周一到周五
Qrcode
Service Center
Contacts
Qrcode
Top
Contacts
Hotline
86-13319518603
Working hours
Monday to Friday
E-mail地址
ivy@hnhbsj.com
Qrcode
Qrcode
添加微信好友,详细了解产品
使用企业微信
“扫一扫”加入群聊
复制成功
添加微信好友,详细了解产品
我知道了
XML 地图