图片展示

HuiBaiYi  New Materias

客服电话:

13319518603

English
  • English
  • 中文
图片展示
图片展示

News

Contact Us

General manager:
+86-731-85525705
+86-13319528603
Fax:+86-731-85525705
E-mail:ivy@hnhbsj.com
Factory Address: Huaihua City, Hunan Province Hongjiang District Industrial Park on the 8th

News Category

Details


Experiment: determination of prednisone

Author:Hunan Hui Bai Yi new materias Co; Ltd Click: Time:2019-12-16 00:00:00

Prednisone is chemically named 11,17, 21-trihydroxyprogesterone 1, 4-diene-3, 20-dione, white or white-like crystalline powder;No odor, slightly bitter taste;There is moisture absorption.Dissolved in methanol or ethanol, slightly dissolved in acetone or dioxane, slightly dissolved in chloroform, very slightly dissolved in water.The molecular formula is C21H28O5, the molecular weight is 360.44400, the density is 1.31 g/cm3, the melting point is 240 °C (dec.)(lit.), and the boiling point is 570.6 C at 760 mmHg.

 

Identify:

(1) take 10mg of this product, add 1ml of methanol to dissolve it, add 1ml of alkaline copper tartrate test solution, and heat it to produce orange-red precipitation.

(2) take about 2mg of this product, add 2ml of sulfuric acid, gradually show deep red color, no fluorescence;Add 10ml water and the red color fades to form gray flocculent precipitate.

(3) in the chromatogram recorded under the content determination, the retention time of the main peak of the sample solution should be consistent with that of the control sample solution.

(4) the infrared absorption spectrum of the product shall be consistent with that of the control group.

 

Content determination:

It was determined by high performance liquid chromatography.

Chromatographic conditions and system suitability test

Using octadecylsilane bonded silica gel as filler;The mobile phase was methanol-water (65:35).The detection wavelength is 240nm.The theoretical plate number should be no less than 1000 according to prednisone peak, and the separation degree between prednisone peak and internal standard material peak should be greater than 3.5.

 

Preparation of internal standard solution:

Norethindrone was dissolved in methanol and diluted to a solution containing approximately 1.5mg per 1ml.

 

Measurement:

Take this product, accurately weigh it, dissolve it with methanol and dilute it quantitatively to make a solution containing about 1mg per 1ml;The solution and the internal standard solution were measured with a precision of 5ml each, placed in a 50ml measuring bottle, diluted with methanol to the scale, shaken well, and then injected into the liquid chromatograph with 10l to record the chromatographic chart.Prednisone was also taken as a reference and determined by the same method.The peak area is calculated according to the internal standard method.

 

The material came from the Internet.

Want to learn more knowledge, biochemical reagent attention please "hunan rhyme state biological pharmaceutical co., LTD." the official website: http://www.hunanyunbang.com/

(all contents of this website, where indicated source is "hunan yunbang", the copyright belongs to hunan yunbang, without authorization, any media, websites or individuals shall not be reproduced, otherwise will be prosecuted for legal responsibility, authorized reproduction must indicate the source: "hunan yunbang".This net indicates the content that the source is other media is to reprint, reprint is for the viewpoint share only, copyright returns original author all, if have infringe copyright, please contact us promptly.


Contact No.:+0731-85525705   +86-13319518603

E-mail: ivy@hnhbsj.com     

Factory Address:  No.8, Hongjiang Industrial Park, Huaihua, Hunan, China

 

Sweep away us

Copyright(C)Hunan Huibaiyi New Materials Co., Ltd. All rights reserved 湘ICP备2022015278号

Service Center
热线电话
400-12346578
上班时间
周一到周五
Qrcode
Service Center
Contacts
Qrcode
Top
Contacts
Hotline
86-13319518603
Working hours
Monday to Friday
E-mail地址
ivy@hnhbsj.com
Qrcode
Qrcode
添加微信好友,详细了解产品
使用企业微信
“扫一扫”加入群聊
复制成功
添加微信好友,详细了解产品
我知道了
XML 地图